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grna cloning vector px330  (Addgene inc)


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    Structured Review

    Addgene inc grna cloning vector px330
    Grna Cloning Vector Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330+cloning+vectors/pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1%2F110)+(Plasmid+%2371707)/pmc10935061-46-1-5
    Average 93 stars, based on 22 article reviews
    grna cloning vector px330 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Synthesized:

    Article Title: Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9.
    Article Snippet: The cells were passaged with accutase (Sigma) after 3 days. .. The FOS sgRNA was designed, synthesized, and cloned to the pX330 cloning vectors (Shinmyo et al., 2016) (Supplementary Fig. S2) (42,230,Addgene). .. Then, 100 million H1 cells were electroporated with 4μg of pX330 plasmid containing sgRNA using the NucleofectorTM 2b Device (Lonza).

    Clone Assay:

    Article Title: Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9.
    Article Snippet: The cells were passaged with accutase (Sigma) after 3 days. .. The FOS sgRNA was designed, synthesized, and cloned to the pX330 cloning vectors (Shinmyo et al., 2016) (Supplementary Fig. S2) (42,230,Addgene). .. Then, 100 million H1 cells were electroporated with 4μg of pX330 plasmid containing sgRNA using the NucleofectorTM 2b Device (Lonza).

    Cloning:

    Article Title: Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9.
    Article Snippet: The cells were passaged with accutase (Sigma) after 3 days. .. The FOS sgRNA was designed, synthesized, and cloned to the pX330 cloning vectors (Shinmyo et al., 2016) (Supplementary Fig. S2) (42,230,Addgene). .. Then, 100 million H1 cells were electroporated with 4μg of pX330 plasmid containing sgRNA using the NucleofectorTM 2b Device (Lonza).



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